Dolomite Bio's Single Cell RNA-Seq System enables high speed encapsulation
Dolomite Bio has developed the Single Cell RNA-Seq System for efficient, high throughput single cell transcriptomics.
Dolomite Bio has developed the Single Cell RNA-Seq System for efficient, high throughput single cell transcriptomics. This compact, scalable system enables rapid, reproducible droplet encapsulation of individual cells, allowing up to 10,000 single cell libraries to be generated in one 15-minute run.
The Single Cell RNA-Seq System allows up to 500 µl samples of cells and oligo-barcoded beads to be encapsulated in extremely monodisperse droplets, each containing a single cell. It incorporates all the components required to encapsulate individual cells, including the hardware - pumps, flow sensors, connectors and a high speed camera and microscope - advanced Flow Control Center software and accessories. The system uses a glass Single Cell RNA-Seq Chip - a commercially available version of the prototype PDMS DropÂseq chip described by Macosko et al1 - offering faster run time, longer life and greater ease of use. Installation and user training is included with each system, ensuring straightforward implementation and rapid initiation of droplet encapsulation experiments. Following encapsulation, the cell is lysed, allowing the mRNA to be captured on the beads for downstream recovery and sequencing.
For more information, please visit, www.dolomite-bio.com/product/rna-seq-system
1 Macosko E et al. (2015). Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets. Cell 161,
About Dolomite Bio
Dolomite Bio creates innovative products for high throughput single cell research. By encapsulating single cells in microfluidic droplets, our products enable rapid analysis of thousands or millions of individual cells and their biological products.
Dolomite Bio is a brand of Blacktrace Holdings Ltd (www.blacktrace.com), a world leader in Productizing Science®. Our Head Office is based in Royston (near Cambridge), UK and we have regional offices in the USA, Japan, India and Brazil.
Dolomite Bios products include μEncapsulator System and RNA Seq system, suitable for use in many applications such as isolating antibody coding sequencing, profiling T cell receptors, droplets for FACS-sorting libraries and encapsulating cells in gel microbeads. To find out more about Dolomite Bio, please visit www.dolomite-bio.com
Featured Product
Cold air to -50°F (-46°C) from your compressed air supply - with no moving parts!
A low cost, reliable, maintenance free solution to a variety of industrial spot cooling problems. Using an ordinary supply of compressed air as a power source, vortex tubes create two streams of air, one hot and one cold, with no moving parts.
Vortex tubes can produce:
• Temperatures from -50° to +260°F (-46° to +127°C)
• Flow rates from 1 to 150 SCFM (28 to 4248 SLPM)
• Refrigeration up to 10,200 Btu/hr. (2571 Kcal/hr.)
Temperatures, flows and refrigeration are adjustable over a wide range using the control valve on the hot end exhaust.
EXAIR Vortex Tubes are constructed of stainless steel. The wear resistance of stainless steel, as well as its resistance to corrosion and oxidation, assures that EXAIR Vortex Tubes will provide years of reliable, maintenance-free operation. https://exair.co/184_530
